Hplc-ms Analysis of the Polyphenols in Two Soft Extracts of Elaeagnus Angustifolia L. Note 2. Soft Extract of Young Branches Analysis
نویسندگان
چکیده
In this paper we aimed to establish the content in polyphenolic compounds (flavones and polyphenolcarboxylic acids) in the soft extract of Elaeagnus angustifolia L. (oleaster) young branches, using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The comparative analysis of the chromatograms obtained before and after hydrolysis showed a complex composition. We identified through MS an unknown compound noted as X, which is a rhamnetol or isorhamnetol heteroside because the pick disappears after hydrolysis. Rezumat In această lucrare s-a urmărit stabilirea conţinutului în compuşi polifenolici (flavone şi acizi polifenolcarboxilici) a extractului moale din ramuri tinere de Elaeagnus angustifolia L., prin cromatografie de lichide de înaltă performanţă cuplată cu spectrometria de masă (HPLC-MS). Analiza comparativă a cromatogramelor obţinute înainte şi după hidroliză a pus în evidenţă o compoziţie complexă. S-a identificat prin MS un compus necunoscut notat X, care este o heterozidă a rhamnetolului sau izorhamnetolului deoarece picul dispare după hidroliză.
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